Abstract
We describe the alteration of protein contents in the phloem sap of rice plants by introduction of transgenes. The coding sequences of rice thioredoxin h (TRXh) and oryzacystatin-I (OC-I) were fused in sense and antisense orientations to the promoter region of the rice TRXh gene. These proteins are present in rice phloem sap. The contents of TRXh and OC-I were reduced in the phloem sap of the transgenic plants carrying the corresponding antisense cDNAs. The content of OC-I protein increased in the phloem sap of transgenic plants carrying the TRXh promoter sense OC-I cDNA. This is the first demonstration of the control of the phloem protein contents in rice plants by analysis of phloem sap of transgenic plants.
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