Abstract
In Arabidopsis thaliana the CPC-like MYB transcription factors [CAPRICE (CPC), TRIPTYCHON (TRY), ENHANCER OF TRY AND CPC 1, 2, 3/CPC-LIKE MYB 3 (ETC1, ETC2, ETC3/CPL3), TRICHOMELESS 1, 2/CPC-LIKE MYB 4 (TCL1, TCL2/CPL4)] and the bHLH transcription factors [GLABRA3 (GL3) and ENHANCER OF GLABRA 3 (EGL3)] are central regulators of trichome and root-hair development. We identified TRY and GL3 homologous genes from the tomato genome and named them SlTRY and SlGL3, respectively. Phylogenic analyses revealed a close relationship between the tomato and Arabidopsis genes. Real-time reverse transcription PCR analyses showed that SlTRY and SlGL3 were predominantly expressed in aerial parts of developing tomato. After transformation into Arabidopsis, CPC::SlTRY inhibited trichome formation and enhanced root-hair differentiation by strongly repressing GL2 expression. On the other hand, GL3::SlGL3 transformation did not show any obvious effect on trichome or non-hair cell differentiation. These results suggest that tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation, and that a CPC-like R3 MYB may be a key common regulator of plant trichome and root-hair development.
Highlights
Epidermal cell differentiation, including trichome and roothair formation, in Arabidopsis thaliana is a popular model system for studying cell fate determination
SlTRY was more closely related to TRY than CPC, which belongs to a cluster that includes TCL1, TCL2/ CPL4, ETC2, and TRY (Figure 1B)
We identified tomato SlTRY and SlGL3 genes that were orthologous to the Arabidopsis TRY and GL3 genes, respectively
Summary
Epidermal cell differentiation, including trichome and roothair formation, in Arabidopsis thaliana is a popular model system for studying cell fate determination. The CAPRICE (CPC) gene encodes an R3 type MYB transcription factor that has been identified as a key regulator of root-hair differentiation [1]. Arabidopsis has several additional CPC-like MYB genes in its genome, including TRYPTICHON (TRY), ENHANCER OF TRY AND CPC1 and 2 (ETC1 and ETC2), ENHANCER OF TRY AND CPC3/CPC-LIKE MYB3 (ETC3/CPL3), and TRICHOMELESS1 and 2/CPC-LIKE MYB4 (TCL1 and TCL2/CPL4) [2,3,4,5,6,7,8,9,10]. The TRY protein has a regulatory role mainly in trichome differentiation [2,11]. TCL1 and TCL2/CPL4 negatively regulate trichome formation on the inflorescence stems and pedicels [8,9,10]
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