Control of mRNA stability contributes to low levels of nuclear poly(A) binding protein 1 (PABPN1) in skeletal muscle

Luciano H Apponi,Anita H Corbett,Grace K Pavlath

doi:10.1186/2044-5040-3-23

Luciano H Apponi, Anita H Corbett + Show 1 more

Open Access

https://doi.org/10.1186/2044-5040-3-23

Copy DOI

Journal: Skeletal Muscle	Publication Date: Oct 1, 2013
Citations: 69	License type: cc-by

Affiliation: Emory University

Abstract

BackgroundThe nuclear poly(A) binding protein 1 (PABPN1) is a ubiquitously expressed protein that plays critical roles at multiple steps in post-transcriptional regulation of gene expression. Short expansions of the polyalanine tract in the N-terminus of PABPN1 lead to oculopharyngeal muscular dystrophy (OPMD), which is an adult onset disease characterized by eyelid drooping, difficulty in swallowing, and weakness in the proximal limb muscles. Why alanine-expanded PABPN1 leads to muscle-specific pathology is unknown. Given the general function of PABPN1 in RNA metabolism, intrinsic characteristics of skeletal muscle may make this tissue susceptible to the effects of mutant PABPN1.MethodsTo begin to understand the muscle specificity of OPMD, we investigated the steady-state levels of PABPN1 in different tissues of humans and mice. Additionally, we analyzed the levels of PABPN1 during muscle regeneration after injury in mice. Furthermore, we assessed the dynamics of PABPN1 mRNA decay in skeletal muscle compared to kidney.ResultsHere, we show that the steady-state levels of both PABPN1 mRNA and protein are drastically lower in mouse and human skeletal muscle, particularly those impacted in OPMD, compared to other tissues. In contrast, PABPN1 levels are increased during muscle regeneration, suggesting a greater requirement for PABPN1 function during tissue repair. Further analysis indicates that modulation of PABPN1 expression is likely due to post-transcriptional mechanisms acting at the level of mRNA stability.ConclusionsOur results demonstrate that PABPN1 steady-state levels and likely control of expression differ significantly in skeletal muscle as compared to other tissues, which could have important implications for understanding the muscle-specific nature of OPMD.

Highlights

The nuclear poly(A) binding protein 1 (PABPN1) is a ubiquitously expressed protein that plays critical roles at multiple steps in post-transcriptional regulation of gene expression
PABPN1 levels are lower in skeletal muscle compared to other tissues A better understanding of the mechanisms that underlie oculopharyngeal muscular dystrophy (OPMD) pathology can be obtained by analyzing the function of PABPN1 in skeletal muscle
Immunoblot analysis revealed that PABPN1 steady-state levels vary significantly among mouse tissues, with skeletal muscle displaying the lowest levels of PABPN1 (Figure 1A)

Summary

Introduction

The nuclear poly(A) binding protein 1 (PABPN1) is a ubiquitously expressed protein that plays critical roles at multiple steps in post-transcriptional regulation of gene expression. RNA-binding proteins regulate all steps of RNA biogenesis and play a key role in post-transcriptional regulation of gene expression [1]. Key players among these RNAbinding proteins are the poly(A)-binding proteins, which modulate 3′-end formation of mRNA transcripts [2]. PABPN1 has critical roles at multiple steps in post-transcriptional regulation of gene expression. PABPN1 is involved in regulation of alternative cleavage and polyadenylation by suppressing weak proximal polyadenylation signals [5], which can influence both gene expression and the structure of the protein produced [6]. PABPN1 modulates a number of processes that are critical for controlling gene expression

Methods

Results

Discussion

Conclusion