Abstract
Several natural isolates of lactic acid bacteria were made from wines which were undergoing spontaneous malolactic fermentation. These organisms were screened for: rapid growth; rapid metabolism of malic acid; and optimal growth response to pH, alcohol, SO<sub>2</sub>, fumaric acid, and temperature. Of the natural isolates studied, a strain designated B44·40 showed the greatest potential for rapid growth, efficient metabolism of malate, and greatest tolerences to variations of pH and temperature. When B44·40 was compared to ML-34 and PSU-1, it was found to grow more rapidly in synthetic media and to metabolize organic substrates present in wine such as malate and glucose at a more rapid rate than either ML-34 or PSU-1. B44·40, ML-34 and PSU-1 showed similar tolerances to alcohol and fumaric acid. B44·40 showed less tolerance to SO<sub>2</sub> than either ML-34 or PSU-1.
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