Abstract
A conditioned medium from a culture of mouse peritoneum showed high activity in stimulating colony formation by mouse bone marrow cells in vitro. In the conditioned medium, two types of activities were separated. The larger molecular component (M fraction) was trypsin-resistant and stimulated macrophage colony formation, and the smaller component (G fraction) was trypsin-sensitive and stimulated granulocyte colony formation. An increased concentration of the G fraction always brought about an increase in granulocyte colonies accompanied by a marked decrease in the number of macrophage colonies. The presence of an inhibitor for macrophage growth is also discussed.
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