Abstract
Publisher Summary Leishmania are pathogenic protozoa that are the etiological agents for leishmaniasis. lipophosphoglycan (LPG) is a multidomain, glycosyl-phosphatidylinositolanchored polysaccharide whose polymorphisms among species lie in the backbone Gal( β 1,4)Man( α 1)-PO4 repeat units and the small oligosaccharide cap.This chapter describes the methods used to isolate purified LPG from Leishmania parasites, and the protocols for obtaining binding information to sand-fly midguts. In the broader view, such methods can be modified and adapted for other glycoconjugate ligands of organisms in interactions with suitable-. The chapter investigates the preparation of procyclic and metacyclic Leishmania promastigotes, extraction and purification of LPG, preparation of delipidated LPG and fragments of LPG, and midgut binding assays of LPG. Developmentally-regulated polymorphisms in LPG structure appear to control the stage specificity of midgut adhesion. A schematic diagram of LPGs from representative procyclic and metacyclic Leishmania species is presented.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
