Control of intestinal bacterial proliferation in regulation of lifespan in Caenorhabditis elegans

Cynthia Portal-Celhay,Martin J Blaser,Ellen R Bradley

doi:10.1186/1471-2180-12-49

Cynthia Portal-Celhay, Martin J Blaser + Show 1 more

Open Access

https://doi.org/10.1186/1471-2180-12-49

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Abstract

BackgroundA powerful approach to understanding complex processes such as aging is to use model organisms amenable to genetic manipulation, and to seek relevant phenotypes to measure. Caenorhabditis elegans is particularly suited to studies of aging, since numerous single-gene mutations have been identified that affect its lifespan; it possesses an innate immune system employing evolutionarily conserved signaling pathways affecting longevity. As worms age, bacteria accumulate in the intestinal tract. However, quantitative relationships between worm genotype, lifespan, and intestinal lumen bacterial load have not been examined. We hypothesized that gut immunity is less efficient in older animals, leading to enhanced bacterial accumulation, reducing longevity. To address this question, we evaluated the ability of worms to control bacterial accumulation as a functional marker of intestinal immunity.ResultsWe show that as adult worms age, several C. elegans genotypes show diminished capacity to control intestinal bacterial accumulation. We provide evidence that intestinal bacterial load, regulated by gut immunity, is an important causative factor of lifespan determination; the effects are specified by bacterial strain, worm genotype, and biologic age, all acting in concert.ConclusionsIn total, these studies focus attention on the worm intestine as a locus that influences longevity in the presence of an accumulating bacterial population. Further studies defining the interplay between bacterial species and host immunity in C. elegans may provide insights into the general mechanisms of aging and age-related diseases.

Highlights

A powerful approach to understanding complex processes such as aging is to use model organisms amenable to genetic manipulation, and to seek relevant phenotypes to measure
Lifespans are shorter when lawns are composed of bacteria that are more pathogenic for humans [21]; host mutations that increase resistance to bacterial infection prolong C. elegans lifespan [22]
Role of downstream immune effector molecules on C. elegans longevity and intestinal bacterial load Since DAF-16 is involved in regulating several antimicrobial proteins and antioxidant enzymes expressed in the intestinal tract [37,38], we addressed the role of the downstream effector molecules

Summary

Introduction

A powerful approach to understanding complex processes such as aging is to use model organisms amenable to genetic manipulation, and to seek relevant phenotypes to measure. Caenorhabditis elegans is suited to studies of aging, since numerous single-gene mutations have been identified that affect its lifespan; it possesses an innate immune system employing evolutionarily conserved signaling pathways affecting longevity. We hypothesized that gut immunity is less efficient in older animals, leading to enhanced bacterial accumulation, reducing longevity. To address this question, we evaluated the ability of worms to control bacterial accumulation as a functional marker of intestinal immunity. Because the nematode Caenorhabditis elegans is genetically tractable, it has become a major model organism for studies of aging [3,4,5], neurobiology [6,7], cell cycle [8], chemosensation [9], microbial pathogenesis, and host defenses [10,11,12].

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