Control of filamentous growth by mating and cyclic-AMP inUstilago

Abstract

Mating between compatible haploid cells of the corn smut fungus, Ustilago maydis, results in a switch from budding to filamentous growth. We are analyzing the multiallelic b incompatibility locus that governs maintenance of the infectious, filamentous dikaryon. Specificity regions have been identified in the N-terminal portions of the bE and bW genes at the b locus. In addition, we have found that heterozygosity at the b locus attenuates fusion. This result suggests that b gene products may exert a negative influence on some processes, in addition to their generally recognized role in maintaining filamentous growth. The b genes have also been characterized in Ustilago hordei. This species has a bipolar mating system in which the b genes are linked to genes required for pheromone production and response to form one large mating-type region (MAT locus) with two allelic specificities. In a separate study, we have discovered that defects in adenylate cyclase result in constitutive filamentous growth and greatly reduce the virulence of U. maydis on corn seedlings. Mutations have been identified that suppress the adenylate cyclase defect and restore budding growth. Sequence analysis revealed that one of the suppressor mutations is in the gene encoding the regulatory subunit of cAMP-dependent protein kinase (PKA). Mutants altered in PKA activity have the interesting phenotype of multiple budding and frequent mislocalization of the bud site. Key words: smut, dimorphism, b locus, sex.