Abstract
The specific activity of NAD pyrophosphorylase (E.C.2.7.7.1) in isolated nuclei of Physarum polycephalum was estimated throughout the cell cycle. The activity of this enzyme increased between 1 and 1 2 h before mitosis and reached a peak at mitosis. The activity remained high throughout the S phase, which follows mitosis directly in this organism and then declined to a stable, basal level which was maintained throughout the G2 period. This fluctuation in activity is similar to that observed for DNA polymerase and thymidine kinase, but is unlike that of other metabolic enzymes in Physarum. The temporal association of the activity of NAD pyrophosphorylase and DNA synthesis suggests a direct integration of NAD metabolism and DNA biosynthesis.
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