Abstract
A stable system for continuous production of acetone and butanol from Clostridium acetobutylicum adsorbed to beechwood shavings has been developed. Different adsorption procedures, influenced by the nutritional conditions in the reactor, were investigated. The cells were kept in a non-growing but active state by a previously developed nutrient dosing technique which also reduced the cell leakage. The system could be operated with maintained activity and very low cell leakage for weeks.
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