Abstract
Bacteriophage-based microbial control could help address a growing need to attenuate the proliferation of antibiotic-resistant bacteria (ARB) in wastewater treatment plants (WWTPs). However, the infectivity of commonly isolated narrow-host-range phages decreases quickly upon addition to activated sludge (i.e., plaque-forming units had a half-life of 0.63 h). Here, we show that polyvalent (broad-host-range) phages proliferate and thrive in activated sludge microcosms, especially when added along with their production hosts. Polyvalent phage cocktails (PER01 and PER02) were significantly more effective than narrow-host-range coliphage cocktails (MER01 and MER02) in suppressing a model ARB [β-lactam-resistant Escherichia coli NDM-1, initially present at 6.2 ± 0.1 log10 colony-forming units (CFU)/mL]. After 5 days, the NDM-1 concentration significantly decreased to 3.8 ± 0.2 log10 CFU/mL in the presence of the polyvalent phage cocktail, compared to 4.7 ± 0.3 log10 CFU/mL for the coliphage cocktail treatment. ...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
