Abstract
Electric pulses, when applied to a cell suspension, induce a reversible permeabilization of the plasma membrane. This permeabilized state is long lived (minutes). The biophysical molecular mechanisms supporting the membrane reorganization associated to its permeabilization remain poorly understood. Modeling them by toroidal lipidic pores cannot explain why they are long lived and why their resealing is under the control of the ATP level. Our results describe the effect of the level of free Calcium ions.
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