Contributions on Spruce Bark Polyphenols Identification Using Instrumental (UV-VIS Spectrometry), Qualitative (Thin Layer Chromatography) and Quantitative (HPTLC Densitometry) Methods

Oana Alexandra Patrautanu,Irina Volf,Oana Teodora Ciuperca,Valentin I Popa

doi:10.37358/rc.20.1.7808

Abstract

This work assessed the qualitative and quantitative polyphenols profile in a crude ethanol extract separated from spruce bark (two fractions with [0.25 and 1 mm diameter) using ultrasound assisted extraction (UAE) as a green extraction method. The evaluation of phenolic acids and condensed tannins profile was performed using instrumental (UV-VIS spectrometry), qualitative (Thin Layer Chromatography, TLC) and quantitative (HPTLC densitometry) methods. Using ultrasound assisted extraction (UAE) technique under specific parameters, the higher total phenolic content (TPC) was 29.785 mg GAE/g-1 for spruce bark fraction with particle size [0.25 mm and 14.448 mg GAE/g-1 for fraction with 1 mm diameters. The TLC assay of the crude ethanol extract was performed considering the standards: gallic, sinapic, p-coumaric and vanillic acids, catechin, epicatechin and tannic acid. Smaller material particle sizes lead to higher yield of polyphenols in ethanol extract. The quantitative evaluation by HPTLC densitometry revealed following amounts: sinapic acid 0.84 mg/g, p-coumaric acid 0.61 mg/g, catechin 1.03 mg/g, tannic acid 2.81 mg/g. Considering the chemical composition, the spread and availability, spruce bark could be considered as a resource with environmental and economic benefits.

Highlights

OANA ALEXANDRA PĂTRĂUŢANU1, OANA TEODORA CIUPERCĂ1,2, VALENTIN I
Ultrasounds incites the formation of tiny bubbles subjected to fast adiabatic compression and expansion, provoking a fast-local increase of temperature and pressure, which leads to a breakage of spruce bark cell wall, the release of intracellular substances, increasing the diffusion across the cell walls and absorption into the solvent [14, 19]
In this study ultrasound assisted extraction (UAE) was conducted at 50°C for 45 minutes using as solvent an ethanol-water (70% v/v) solution in a solid/liquid (S/L) ratio of 1:10 g sample/mL solvent

Summary

Introduction

OANA ALEXANDRA PĂTRĂUŢANU1, OANA TEODORA CIUPERCĂ1,2, VALENTIN I. This work assessed the qualitative and quantitative polyphenols profile in a crude ethanol extract separated from spruce bark (two fractions with

Methods

Results

Conclusion