CONTRIBUTION TO MOLECULAR GENETIC CHARACTERIZATION OF HELLEBORUS MULTIFIDUS Vis. IN BOSNIA AND HERZEGOVINA

Lejla Lasić,Semir Dorić,Jasna Hanjalić,Jasmina Čakar,Gabrijela Radosavljević,Naris Pojskić,Belma Kalamujić Stroil

doi:10.54652/rsf.2016.v46.i2.71

Abstract

UDK: 582.675.1:575(497.6) Helleborus multifidus Vis. is endemic Illyric-Adriatic species with distribution range in Italy, Slovenia, Croatia, Bosnia and Herzegovina, Montenegro and Albania. Although few studies reported different taxonomic categories for H. multifidus, this one is the first using molecular-genetic markers (trnL region and matK of chloroplast DNA and nuclear ITS1 and ITS2 region) for genetic characterization of H. multifidus presented at three localites in Bosnia and Herzegovina. The results revealed that PCR-RFLP on trnL intron was not informative for testing inter- or intrapopulation diversity. Contrary, analysis of matK, ITS1 and ITS2 sequences showed differences between populations from Trebinje region and Kupreško polje, pointing to the need to include additional analyses in order to confirm these findings.

Highlights

Helleborus multifidus Vis. is endemic Illyric-Adriatic species with distribution range in Italy, Slovenia, Croatia, Bosnia and Herzegovina, Montenegro and Albania
The number of species and subspecies varies from 11 species (TUTIN, 1996), 15 species and 10 subspecies (MATHEW, 1989; ZONNEVELD, 2001) to 22 species based on recent taxonomic reviews (MEINERS ET AL., 2011)
The aim of this study was genetic characterization of H. multifidus collected at three localities in Bosnia and Herzegovina using molecular variation of trnL region of chloroplast DNA, matK, ITS1 and ITS2 markers

Summary

Introduction

Helleborus multifidus Vis. is endemic Illyric-Adriatic species with distribution range in Italy, Slovenia, Croatia, Bosnia and Herzegovina, Montenegro and Albania (MARTINIS, 1973). The aim of this study was genetic characterization of H. multifidus collected at three localities in Bosnia and Herzegovina using molecular variation of trnL region of chloroplast DNA, matK, ITS1 and ITS2 markers. Sampling of plant material – Uzorkovanje biljnog materijala Plant material of 110 hellebore specimens was collected during the plant flowering phase (April-June 2015) from three different localities in Trebinje region (locality 1 - 43°09'79'' N, 18°24'52" E and locality 2 - 43°11'57'' N, 18°24'07" E) and Kupreško polje (locality 3 - 43°94'99'' N, 17°20'32" E) (Figure 1).

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Conclusion