Contribution to interpretation of metal uptake dependence upon the growth phase of microorganisms. The case of uranium (VI) uptake by common yeasts, cultivated at different temperatures, with or without aeration

Abstract

The dependence of U(VI) uptake on the temperature of cell culture, the air flow during the cultivation process and the age of cells were studied. Saccharomyces cerevisiae, Kluyveromyces marxianus and Debaromyces hansenii were chosen as typical yeasts, which are widely used, in food industries. Our results revealed that the highest metal uptake was obtained from exponential phase cells, which had been cultivated at the optimum temperature of growth, while the air flow during the cultivation process, exhibited no significant effect on the metal uptake. A qualitative interpretation of bibliographic data, concerning the metal uptake on the age of cells is proposed, assuming that qualitative changes in the cell wall structure take place, as the cells pass from exponential to stationary phase, in addition to quantitative modifications, which have been reported in the literature. According to our interpretation, the relative abundances among quantitative and qualitative alterations of cell wall, determine which cells (exponential or stationary) exhibit the higher metal capacity. One type of the suggested qualitative modifications of surface constituent of cell wall, may have been caused by a shortening of a carboxylic acid carbon chain. This type of modification implies, as prerequisite, the decrease of pK a values of cell wall carboxyl groups, with the age of cells. An evidence, supporting our approach, may be the fact that the decrease of pK a values mentioned above, has been observed by other authors.