Contribution of yersiniabactin to the virulence of an Escherichia coli sequence type 69 (“clonal group A”) cystitis isolate in murine models of urinary tract infection and sepsis

Abstract

Escherichia coli sequence type 69 (ST69; “clonal group A”) is an important extraintestinal pathogen. To clarify the yersiniabactin siderophore system's role in ST69's extraintestinal virulence we compared a wild-type ST69 cystitis isolate, isogenic irp2 (yersiniabactin) mutants, and irp2-complemented mutants in murine models of sepsis and urinary tract infection (UTI). irp2 mutants were attenuated mildly in the UTI model and profoundly in the sepsis model. In both models, complementation with a functional copy of irp2 restored full parental virulence. These findings suggest that in ST69 the yersiniabactin system has a minor role in urovirulence and a major role in sepsis causation.