Abstract
Spermatogenic immunoglobulin superfamily (SgIGSF) is a recently identified adhesion molecule, and the microphthalmia transcription factor (MITF) was essential for its expression in mast cells. Since the tg mutant allele is practically a null mutation of the MITF gene, cultured mast cells (CMCs) derived from (WB × C57BL/6)F 1 (F 1)- tg/tg mice did not express SgIGSF whereas CMCs from F 1-wild-type (+/+) mice expressed it abundantly. When cocultured with NIH/3T3 fibroblasts, F 1- tg/tg CMCs showed poor adhesion to NIH/3T3 fibroblasts. When injected intraperitoneally, F 1- tg/tg CMCs showed poor survival in the peritoneal cavity of mast cell-deficient F 1- W/ W v mice. SgIGSF was expressed in tg/tg CMCs ectopically through retroviral transfection and through expression of a transgene. The resulting tg/tg CMCs showed not only a better adhesion to NIH/3T3 fibroblasts but also a better survival in the peritoneal cavity than control F 1- tg/tg CMCs. SgIGSF-mediated adhesion seemed to play a role in the survival of CMCs in the peritoneal cavity.
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