Abstract
BackgroundFirst ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. In people older than 18 months of age, serological confirmation test is recommended to confirm HIV infection.Case presentationHere we report the case of a patient tested positive with HIV-1, ELISA, Murex® Ag⁄Ab Combination assay (OD450 = 0.802 and cutoff-OD = 0.279) and negative by using FIRST RESPONSE HIV1-2.O CARD TEST (version 2.0) RAPID HIV CARD TEST. Viral load performed with Cobas® TaqMan® 96/Cobas® Ampliprep® was 6.49log10. The virus could be sequenced in partial gag and pol genes and belonged to CRF02_AG clade.ConclusionConventional PCR is a complementary method for the diagnosis of inconclusive HIV-1 serologies by antibodies.
Highlights
First ambitious target by 2020 of United Nations Pro‐ gramme on Human immunodeficiency viruse (HIV)/Acquired immune deficiency syndrome (AIDS) (UNAIDS) is that 90% of people living with HIV know their HIV status
The first ambitious target by 2020 of United Nations Pro‐ gramme on HIV/AIDS (UNAIDS) is that 90% of people living with HIV know their HIV status
A 25-year old man was referred to HIV confirmation test in National Laboratory Fighting Against AIDS (LNR/ Health program fighting against AIDS in Benin (PSLS)) in Benin for HIV detection because his HIV infection diagnosis was indeterminate in remote area laboratory, albeit he had no clinical symptoms
Summary
First ambitious target by 2020 of UNAIDS is that 90% of people living with HIV know their HIV status. The use of DNA and RNA PCR is essential in case of low level of antibodies during the window period or at the final stage of HIV infection or in the acute phase of infection [1]. We report the case of a patient with indeterminate results in antibodies HIV-1 serology but positive for classical RNA PCR.
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