Abstract
The differences in C isotope ratio of C<sub>3</sub> and C<sub>4</sub> plant species have been used to determine relative contributions of carbon (C) sources to total CO<sub>2</sub> efflux. The objective of this study was to estimate the contribution of soil organic C and C<sub>3</sub> sugar to total CO<sub>2</sub> of corn and wheat monocultures during a short-term incubation. Control soils and soils amended with sugar were incubated at 25&deg;C for 48 hours and total CO<sub>2</sub> concentration and &delta;<sup>13</sup>C values of evolved CO<sub>2</sub> were measured. The proportional contribution of C sources on CO<sub>2</sub> efflux was determined by using isotopic composition of soil organic C and C<sub>3</sub> sugar. &delta;<sup>13</sup>C values of soils are highly affected by the type of vegetation and the soil management. The C<sub>3</sub> sugar addition in soils double the CO<sub>2</sub> efflux in the corn soil, but it did not affect CO<sub>2</sub> efflux in the wheat soil. This indicated a larger turnover of microbial biomass in the corn soil. The greatest significant (P &lt; 0.05) difference in &delta;<sup>13</sup>C values between the control and sugar added soils occurred at 12 hours in the corn soil (11.2&permil;) and at 24 hours in the wheat soil (9.4&permil;). The estimated relative contribution of sugar to CO<sub>2</sub>efflux was stronger at 12 hours incubation in the corn soil.
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