Contribution of perfusion to the 11 C-acetate signal in brown adipose tissue assessed by DCE-MRI and 68 Ga-DOTA PET in a rat model.

Abstract

Determine if dynamic contrast enhanced (DCE) -MRI and/or 68 gallium 1,4,7,10-tetraazacyclododecane N, N', N″, N‴-tretraacetic acid (68 Ga-DOTA) positron emission tomography (PET) can assess perfusion in rat brown adipose tissue (BAT). Evaluate changes in perfusion between cold-stimulated and heat-inhibited BAT. Determine if the 11 C-acetate pharmacokinetic model can be constrained with perfusion information to improve assessment of BAT oxidative metabolism. Rats were split into three groups. In group 1 (N=6), DCE-MRI with gadobutrol was compared directly to 68 Ga-DOTA PET following exposure to 10°C for 48h. 11 C-Acetate PET was also performed to assess oxidation. In group 2 (N=4), only 68 Ga-DOTA PET was acquired following exposure to 10°C for 48h. Finally, in group 3 (N=10), perfusion was assessed with DCE-MRI in rats exposed to 10°C or 30°C for 48h, and oxidation was measured with 11 C-acetate. Perfusion was quantified with a two-compartment pharmacokinetic model, while oxidation was assessed by a four-compartment model. DCE-MRI and 68 Ga-DOTA PET provided similar perfusion measures, but a decrease in the perfusion signal was noted with longer imaging sessions. Exposure to 10°C or 30°C did not affect the perfusion measures, but the 11 C-acetate signal increased in BAT at 10°C. Without prior information about blood volume, the 11 C-acetate compartment model overestimated blood volume and underestimated oxidation in 10°C BAT. Precise assessment of oxidation via 11 C-acetate PET requires prior information about blood volume which can be obtained by DCE-MRI or 68 Ga-DOTA PET. Since perfusion can change rapidly, simultaneous PET-MRI would be preferred.