Abstract
Inherited variance in the IL-12B gene is associated with susceptibility to Chlamydia trachomatis-induced tubal factor infertility and disease severity. In this study, our aim was to discover how polymorphisms in IL-12-coding genes influence C.trachomatis-induced immune responses and IL-12 production. The study population consisted of 240 women. IL-12A and IL-12B single nucleotide polymorphisms (SNPs) were determined from isolated DNA using the Sequenom system with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. We studied lymphocyte proliferative (LP) responses to C.trachomatis strains E and F elementary bodies (EBs) and recombinant chlamydial heat-shock protein 60 (CHSP60) antigen. IL-12p40 and IL-12p70 levels were measured using the BD Flex Set method. We found a statistically significant association between the C.trachomatis EB antigen-specific LP response and the rs2853694 SNP (P=0.02). Our study demonstrates that the IL-12 cytokine family is involved in C.trachomatis-specific immune responses. Moreover, C.trachomatis-induced IL-12 production and the IL-12B rs2853694 SNP partially explain individual variation in the C.trachomatis LP response.
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