Contribution of flow cytometry to estimate picoplankton biomass in estuarine systems

Abstract

Picoplankton (plankton ≤3 μm) biomass was determined by flow cytometry in three European estuarine systems (Krka Estuary in Croatia, Rhone Delta in France, and Lena Delta and Laptev Sea in Russia). The size of natural phytoplankton groups was obtained by a calibration curve, with different picoplankton's strains (from 1.6 to 3.4 μm), measured by a Coulter counter (size) and a flow cytometer (light-scattering). Two natural groups of picoplankton were identified by flow cytometry in the three systems: Synechococcus sp and picoeukaryotes. Picoplankton cells abundance ranged between: 2800 and 42 000, 5000 and 37 000, 1000 and 50 000 cells ml−1 in the Krka estuary, in the Rhone delta and in the Lena-Laptev system, respectively. In the Krka estuary, picoplankton biomass ranges between 11 and 68 μgC l−1. It can make up as much as 88% of the total photosynthetic plankton population and 50% of total organic particulate carbon. Picoplankton biomass was greater in the summer than in the autumn. At the halocline layer this biomass can attempt ca. 390 μgC l−1during the summer cruise. In the Rhone delta, a lower picoplankton biomass (6–39 μgC l−1) was observed at the end of the winter. These biomass represented between 0.4 and 22% of the particulate organic carbon, which could reach 71% of the total photosynthetic plankton biomass at the marine station. In the Lena-Laptev system, picoplankton biomass varied between 6 and 56 μgC l−1 in surface waters. Picoplankton biomass decreased with depth, but picoeukaryotes were still observed in deep samples (20, 30 m) in the Laptev Sea, showing a considerable autotrophic activity in spite of low temperatures (0–1 °C). Although the widely dispersed estuary geographic distribution and their different estuarine characteristics, the data point out that these small organisms can also play an important role in the transfer of organic carbon from rivers to oceans and that flow cytometry can be able to detect these small cells in turbid systems.