Contribution of Bacillus cereus ERBP in ozone detoxification by Zamioculcas zamiifolia plants: Effect of ascorbate peroxidase, catalase and total flavonoid contents for ozone detoxification

Abstract

Eighteen plant species were screened for ozone (O3) removal in a continuous system. Zamioculcas zamiifolia had the highest O3 removal efficiency. To enhance O3 removal by Z. zamiifolia, adding a compatible endophytic bacteria, Bacillus cereus ERBP into Z. zamiifolia was studied. After operating under an O3 continuous system (150–250 ppb) at a flow rate of 0.3 L min−1 for 80 h, inoculated plants (74%) exhibited higher O3 removal efficiency than non-inoculated ones (53%). In addition, after O3 exposure (80 h), the population of B. cereus ERBP in inoculated plants was significantly increased in both shoots approximately 35 folds and leaves 13 folds compared to inoculated plants without O3 exposure. The results also showed that B. cereus ERBP had the ability to protect Z. zamiifolia against O3 stress conditions. The increase in B. cereus ERBP populations was attributed to the significant increase in ascorbate peroxidase (APX) and catalase (CAT) activity. In addition, increasing B. cereus ERBP populations led to raise total flavonoid contents which is one of antioxidant compounds. Increasing APX, CAT activities, and total flavonoid contents can enhance O3 detoxification in plant tissues. The mechanism of B. cereus ERBP for enhancing O3 phytoremediation was proposed in this study. The results suggested that B. cereus ERBP was a potential tool for alleviating O3 stress on Z. zamiifolia and enhancing O3 phytoremediation efficiency.