Contributed Session III: In vivo calcium imaging of macaque foveolar retinal ganglion cells reveals spatiochromatic receptive field properties.

Abstract

Here, we optically record responses to spatial and chromatic stimuli using a calcium indicator in the living macaque eye to characterize the receptive field (RF) properties of retinal ganglion cells (RGCs) serving the foveal center. GCaMP6s was expressed in three female macaques. Adaptive optics ophthalmoscopy was used to image fluorescence (488nm ex, 520/35nm em) from RGCs whose RF centers were driven by cones in the central 36 arcmin of the fovea and additional RGCs driven by cones in the central 6 arcmin of the foveola. Using cone isolating and luminance flicker (1.3deg, 0.15Hz, LED 420nm, 530nm, 660nm), we derived cone weights in over 250 RGCs. Using drifting gratings (1.9deg, 6Hz, 4-50c/deg, 561nm), we derived the spatial frequency responses of 15 L vs. M cone opponent RGCs at the foveolar center. Employing computational modeling (ISETbio), we inferred the full spatial difference of gaussians center and surround structure for those 15 cells. Of the 34 foveolar RGCs, 44% exhibited L vs. M cone opponency, 15% were L+M ON, 6% were -L-M OFF, and 35% showed only L or only M responses. The spatial frequency response functions of 12/15 L vs. M opponent cells peaked at high spatial frequencies (25-40c/deg) and had a strong bandpass characteristic. Our model indicates that the responses of all 15 L vs. M opponent cells are consistent with single cone input to their RF centers and that our data are consistent with extrafoveal data when the blurring of the optics is accounted for.