Contrast ultrasound imaging of the aorta does not affect progression of atherosclerosis in ApoE−/− mice

Abstract

Ultrasound Contrast Agents (UCAs) are used clinically to enhance ultrasound imaging of the cardiovascular system. Adverse biological effects have been noted after administration of UCAs, and more research is needed for an understanding of the biological effects of UCAs. Male ApoE−/− mice (8 weeks old, n=38) were intravenously infused with the Definity® UCA (2×1010 UCA/hr) and either exposed to 2.8‐MHz 10‐Hz PRF 1.4‐μs PD 2‐min ED 1.4‐MPa PRPA ultrasound or sham exposed, and then consumed either a chow or Western diet for either 4 or 8 weeks after ultrasound exposure (n=4–5 per group). Plasma total cholesterol was greater in the Western diet group than the chow group for all timepoints after baseline (p<0.0001). Plasma von Willebrand Factor (vWF), a biomarker of endothelial function, was elevated in Western diet animals compared to chow animals (p<0.05). Atheroma thickness was greater in animals consuming the Western diet than in chow‐fed animals (p<0.001), and in animals euthanized after 8 weeks than after 4 weeks (p<0.005). Ultrasound did not affect plasma total cholesterol levels, plasma vWF or atheroma thickness. [Supported by NIH R37EB002641]