Abstract
The contrast of particles of colloidal gold in the backscattered electron mode of high-resolution low-voltage scanning electron microscopy is of interest for the use as markers in surface labeling techniques of electron microscopical histochemistry. Monte Carlo calculations show a contrast maximum when the particle diameter is comparable with the electron range in gold, and the electron energy showing the maximum decreases with decreasing diameter. The calculations are confirmed experimentally by measuring the contrast of 40 nm particles of colloidal gold and evaporated gold films on a silicon substrate for comparison.
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