Contradictory between morphology and phylogenetic trees of Orthosiphon spp. (Lamiaceae) from Indonesia

Abstract

The nuclear ribosomal Internal Transcribed Spacer region (ITS), and three chloroplast loci (trnL-trnF, rps16 and trnL) were also carried out in this study. Morphological analysis of leaves, stems, and flowers is used to identify visual differences. This study was the purpose of analyzed morphological and phylogenetic relationships among ten taxa of Orthosiphon from the analysis of DNA sequences. The results that the difference in leaves, flowers and stems is very striking in seven taxa of Orthosiphon aristatus (OGP and OGW) and O. endanghidayatae (OWW, OG + W and OW + W). The phylogenetic trees constructed from cpDNA (trnL gene, trnL-trnF intergenic spacer region, and rps16 region) of using NJ methods among taxa of Orthosiphon aristatus and O. endanghidayatae collected from Indonesia showed constant topologies with high bootstrap values (BS 86% and 100%, respectively), but were different from those ITS of nrDNA that the phylogenetic trees supported low bootstrap values (65% by NJ). Although differing from morphology to species of Orthosiphon spp. but still similar in DNA analysis. The results of this study indicate that the speciation process of O. endanghidayatae (OWW, OG + W and OW + W) is as a process of morphological changes faster than the process of genetic change.