Contraction of human airways by oxidative stress: Protection by n-acetylcysteine

Abstract

We examined the in vitro effects of tert-butylhydroperoxide (tBu-OOH) in human bronchial muscle. tert-Butylhydroperoxide produced concentration-dependent contractions of bronchial rings (maximum effect was 56.5 ± 9.6% of contraction by 1 mM acetylcholine; effective concentration 50% was ∼100 μM). tert-Butylhydroperoxide (0.5 mM)-induced contraction was enhanced by epithelial removal but abolished by indomethacin (cyclooxygenase inhibitor) and zileuton (lipoxygenase inhibitor). tert-Butylhydroperoxide produced a transient rise in intracellular calcium in human cultured airway smooth muscle cells (HCASMC). The bronchial reactivity to acetylcholine and histamine was not altered by tBu-OOH. In HCASMC, tBu-OOH (0.5 mM, 30 min) increased malondialdehyde levels (MDA; from 7.80 ± 0.83 to 26.82 ± 1.49 nmol mg −1 protein), accompanied by a decrease of reduced glutathione (GSH; from 16.7 ± 2.6 to 6.9 ± 1.9 nmol mg −1 protein) and an increase of oxidized glutathione (from 0.09 ± 0.03 to 0.18 ± 0.03 nmol mg −1 protein). N-acetylcysteine (0.3 mM) inhibited by approximately 60% the bronchial contraction resulting from tBu-OOH (0.5 mM) and protected cultured cells exposed to tBu-OOH (MDA was lowered to 19.51 ± 1.19 nmol mg −1 protein, and GSH content was replenished). In summary, tBu-OOH caused contraction of human bronchial muscle mediated by release of cyclo-oxygenase and lipoxygenase products without producing airways hyperreactivity. N-acetylcysteine decreases tBu-OOH-induced contraction and protects human cultured airway smooth muscle cells exposed to tBu-OOH.