Abstract
Contractile skeletal muscle cells were cultured so as to wrap around an electrode wire to enable their selective stimulation even when they were co-cultured with other electrically-excitable cells. Since the electrode wire was composed of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU), which is soft and highly capacitive (~10 mF cm−2), non-faradaic electrical stimulation with charge/discharge currents could be applied to the surrounding cells without causing significant damage even for longer periods (more than a week). The advantage of this new culture system was demonstrated in the study of chemotactic interaction of monocytes and skeletal muscle cells via myokines.
Highlights
In the previous study[19,20], we utilized a polymer composite consisting of conducting polymers poly(3, 4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU) to fabricate a soft organic electrode
The PEDOT/PU composite electrode retains large surface capacitance (~10 mF cm−2)[20]. This soft and highly capacitive electrode was processed into twisted fine wire, and enveloped in a layer of contractile myotubes to form a skeletal muscle bundle (Fig. 1). The advantage of this new culture system was demonstrated by selective stimulations of the skeletal muscle bundle to study contraction-dependent endocrine effects of myokines on the activity of co-cultured monocytes
The PEDOT/PU wire was insulated with virgin PU film, except the conductive area of 5 mm in length, indicated by the blue double-headed arrow (Fig. 2a)
Summary
In the previous study[19,20], we utilized a polymer composite consisting of conducting polymers poly(3, 4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU) to fabricate a soft organic electrode Composite electrode) that conformed to active motion of the tissue. The PEDOT/PU composite electrode retains large surface capacitance (~10 mF cm−2)[20]. In this study, this soft and highly capacitive electrode was processed into twisted fine wire, and enveloped in a layer of contractile myotubes to form a skeletal muscle bundle (Fig. 1). This soft and highly capacitive electrode was processed into twisted fine wire, and enveloped in a layer of contractile myotubes to form a skeletal muscle bundle (Fig. 1) The advantage of this new culture system was demonstrated by selective stimulations of the skeletal muscle bundle to study contraction-dependent endocrine effects of myokines on the activity of co-cultured monocytes
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