Abstract
The purpose of this paper is to review our previous work with HMM binding and to demonstrate with some new findings that this technique can be used effectively to determine changes in the organizational states of the actin-like microfilaments which are responsible for cytoplasmic streaming in laticifer cells and for conduction of the stigmotropic stimulus in the cytoplasm of mature sieve elements. The species used were Mimosa rixosa Mart., M. asperata Linn., and Mimosa laticifera Rizz.The technique used was negative coloration of phloem exudate and cytoplasmic suspension of latex. We have identified actin filaments in phloem exudate and latex of higher plants on the basis of filament morphology, reaction of the filaments with rabbit skeletal muscle HMM to form arrowhead complexes, and the reversibility of the arrowhead reaction on addition of pyrophosphate. This is the first report dealing with the presence of actin in sieve elements. Some of the microfilament structures are specific components of the cytoplasm of mature sieve tubes.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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