Continuous sheathless microparticle and cell patterning using CL-SSAWs (conductive liquid-based standing surface acoustic waves)

Jeonghun Nam,Jae Young Kim,Chae Seung Lim

doi:10.1063/1.4975397

Jeonghun Nam, Jae Young Kim + Show 1 more

Open Access

https://doi.org/10.1063/1.4975397

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Journal: AIP Advances	Publication Date: Jan 1, 2017
Citations: 10	License type: cc-by

Affiliation: Korea University Medical Center, Korea University

Abstract

We present continuous, sheathless microparticle patterning using conductive liquid (CL)-based standing surface acoustic waves (SSAWs). Conventional metal electrodes patterned on a piezoelectric substrate were replaced with electrode channels filled with a CL. The device performance was evaluated with 5-μm fluorescent polystyrene particles at different flow rate and via phase shifting. In addition, our device was further applied to continuous concentration of malaria parasites at the sidewalls of the fluidic channel.

Highlights

Microfluidic techniques for manipulating microparticles have become critical for various biochemical studies and clinical applications, such as flow cytometry, tissue engineering, cell sorting and separation, and disease diagnosis.[1,2,3] In these applications, microparticles are required to be arrayed in either a one-dimensional pattern or a two-dimensional pattern for accurate detection and sorting.[4]
The analysis location was at the end of the conductive liquid (CL)-standing surface acoustic waves (SSAWs) working region, which was 3 mm downstream from the onset of the CL-based standing SAW (CL-SSAW) field
The maximum flow rate that allowed tight patterning along the pressure nodes with an input power of 320 mW was 3 μL/min, which corresponds to a minimum required time in the CL-SSAW working region of ∼ 290 ms

Summary

Introduction

Microfluidic techniques for manipulating microparticles have become critical for various biochemical studies and clinical applications, such as flow cytometry, tissue engineering, cell sorting and separation, and disease diagnosis.[1,2,3] In these applications, microparticles are required to be arrayed in either a one-dimensional pattern or a two-dimensional pattern for accurate detection and sorting.[4]. Electrode channels have been the most promising substitute for patterned metal electrodes in various electric microfluidic devices, including electrophoretic and dielectrophoretic applications.[18,19,20] channel-based threedimensional electrodes can generate a uniform electric field across the microchannel, the applicability of these techniques for microparticle manipulation can be limited because the device performance depends on the electrical properties of the microparticles and the suspending medium. Acoustic methods manipulate target particles depending on relative physical properties (size, deformability, density, etc.) in a non-invasive way and are more versatile than electrical fieldbased techniques for various applications, including focusing, patterning, separation and enrichment

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