Abstract

Continuous forms of chromatography are important to the biotechnology industry for the production of therapeutic proteins. Selective non-adsorption preparative (SNAP) chromatography may be used in a cross-current, simulated moving bed to produce a continuous purification system. It has the advantages of speed, efficient use of packing material, scalability, decrease cost of pumping systems, gentleness with regard to protein denaturation, and reduced dilution of the sample. An immobilized metal affinity chromatography (IMAC) column may be added in tandem with SNAP to concentrate as well as purify the protein.

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