Abstract
Indole acetic acid (IAA) is a plant growth-promoting hormone used in agriculture; therefore, its continuous production is of paramount importance. IAA-producing eight bacteria were isolated from the rhizosphere of Verbascum vulcanicum. Among them, Arthrobacter agilis A17 gave maximum IAA production (75 mg/L) and this strain was used to immobilization studies. The A. agilis A17 cells were immobilized in calcium alginate for the production of IAA. Optimization of process parameters for IAA production was carried out to enhance IAA production using immobilized cells. The maximal production of IAA was 520 mg/L under the following optimal conditions: 1% mannitol, 30 °C, pH 8.0, and 24 h incubation. It was determined that the immobilized cells could be reused (13 times) for the production of IAA.
Highlights
Indole acetic acid (IAA) is a natural auxin which is produced by plants, bacteria and fungi
From the eight isolates obtained during screening, isolate A17 gave the best result for IAA production
Nucleotide sequences were compared with National Center for Biotechnology Information (NCBI) GenBank entries and the similarities were determined by the BLAST algorithm (Fig. 1)
Summary
Indole acetic acid (IAA) is a natural auxin which is produced by plants, bacteria and fungi. Many researchers reported that plant growth-promoting bacteria from different genera (Azospirillum, Bacillus, Enterobacter, Azotobacter, Klebsiella, Pseudomonas, and Arthrobacter), Actinomycetes (Streptomyces olivaceoviridis, S. rimosus) and fungi (Colletotrichum gloeosporioides, Ustilago maydis) enhance plant growth by the synthesis of IAA (Khamna et al 2010; Reineke et al 2008). Immobilized biocatalysts are widely used to produce different types of products and enzymes. They provide many benefits, such as higher stability, lower operational costs, continuous use of the biocatalysts, higher resistance to contamination, easier separation from the production medium and enhanced reaction yield (Kurbanoglu et al 2010a, b; Okay et al 2013). There is no earlier study using Ca-alginate immobilized A. agilis cells for IAA
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