Abstract
AbstractA glucose‐limited chemostat was used to determine the growth parameters of BBK006 for continuous production of the biosurfactant surfactin. The continuous cultivation exhibited low maintenance metabolism (m = 0.39 mmolglucose gbacteria−1 h−1) and high molar growth yield ($Y_{x/S}^{\max} = 30.8$ gbacteria molglucose−1). It was found that the surfactin production rate in continuous culture was not only a function of dilution rate but also varied with the initial concentration of glucose in the feed. A high steady state concentration of surfactin (18 mg L−1) was maintained in the culture at a dilution rate of 0.2 h−1 when glucose concentration in the feed was 0.25 g L−1. This is the first demonstration of continuous surfactin production and recovery using glucose as a carbon source. The production of surfactin is known to be related to the age of the microorganisms and a simple mathematical model has been constructed to show how the age‐related production can be quantified. Copyright © 2006 Society of Chemical Industry
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