Abstract
Cyclodextrin glycosyltransferase (CGTase) from Bacillus sp. C26 was highly stable when it was immobilized in mixed alginate–gelatin gel beads. Enzymatic synthesis of β-cyclodextrin (β-CD) from starch by immobilized CGTase was performed in a continuous stirred-tank reactor (CSTR) and a packed-bed reactor (PBR). A comparative study on reaction kinetics found that the apparent maximum production rate (Vmax,app) for reaction in CSTR (0.5742gL−1h−1) was higher than that in PBR (0.33gL−1h−1) likely due to the higher mass transfer rate in CSTR. The lower apparent Michaelis–Menten constant (Km,app) in PBR (31.88gL−1) compared to that in CSTR (58.3gL−1) indicate that the enzyme in PBR required lower amount of substrate to become half-saturated. The appropriate dilution rate for operation in both reactors was the same at 0.75h−1. To effectively produce β-CD, an integrated CSTR-PBR was developed. Through this integrated system, the final β-CD concentration and yield were improved from 6.10gL−1 and 15.3% in a single CSTR up to 10.6gL−1 and 26.5%, respectively. Moreover, the immobilized CGTase had good operational stability for 96h of continuous use. This study has shown that the integrated CSTR-PBR was effective for continuous production of β-CD from starch and may greatly contribute to developing industrialized production of β-CD.
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