Abstract
Photofermentative hydrogen production applying Rhodobacter sphaeroides O.U.001 immobilized on porous glass plates or porous and non-porous glass beads in presence of malic acid (Biebl and Pfennig medium) was studied under continuous and semicontinuous conditions. The applied bacteria operating under continuous regime showed the best performance and stability while immobilized on porous glass plate modified with (3-(2-aminoethyl)aminopropyl)trimetoxysilane (AEAPTMS). Here, the total amount of produced hydrogen was 4.19 L/reactor whereas average hydrogen production rate was 12.7 mL/L medium/h at hydraulic retention time (HRT) of 22 h. For experiments in suspension cumulative hydrogen amount was only 2.39 L/reactor. Accumulation of VFA's (mainly formic acid) in reaction medium as well as accumulation of biomass on supports was responsible for the decreasing activity. Comparison of different parameters in experiments with porous and non-porous glass beads proved that HRT plays a crucial role in photofermentative hydrogen production.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
