Continuous Measurement of Negative Pressure Effects on Cell Migration in Wounded Monolayer Cell with Electrical Wound‐Healing Assay

Abstract

BackgroundNegative pressure wound therapy has recently gained popularity in chronic wound care. This study attempted to explore effects of different negative pressures on cell migration in wound healing process.MethodsWe used the electric cell‐substrate impedance sensing technique to create a 5×10−4 cm2 wound in the monolayer Madin‐Darby Canine Kidney cells and continuously monitor cell migration activities. The wounded cells were cultured in a self‐constructed negative pressure incubator at ambient pressure and negative pressures of 75 mmHg, 125 mmHg and 175 mmHg. The effective time, complete wound healing time, healing rate, cells radius and wound area over time were evaluated.FindingsThe effective time (1.25±0.27 hrs), complete healing time (1.76±0.32 hrs), healing rate (2.94±0.62 cm2/hr), cell radius (11.5±0.2 μm) at complete wound healing of cells treated at negative pressure of 125 mmHg were significantly different from those at other three pressure conditions.InterpretationThe study has established a cellular model for observing cell migration at different negative pressures uninterruptedly. Results have shown that the negative pressure of 125 mmHg can enhance cell migration activites.