Abstract
Somatic embryogenic callus was induced on two induction media, B5h and SH4K. Embryos formed on the callus induced on B5h medium when the callus was still on the induction medium. On the other hand, embryos could not form on the callus induced on SH4K medium unless the callus was transferred to a growth regulator-free medium. Callus induced and maintained on B5h medium lost embryogenic capability quickly during the subculture. Callus induced and maintained on SH4K medium, however, consistently remained highly embryogenic. The callus mass showed steady increase during its maintenance on SH4K medium. The embryos induced on SH4K medium showed vigorous germination. Normal and fully fertile plants were recovered from the embryos developed from the callus maintained on SH4K medium.
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