Abstract

A novel disk photo-bioreactor was employed to produce hydrogen continuously by Rhodopseudomonas faecalis RLD-53. The ability of hydrogen production was investigated in different feeding times and HRTs. The maximum total hydrogen yield in the photo-bioreactor reached 2.68 mol H2/mol acetate at 48h HRT and 4d feeding time. Experimental results indicated that feeding times and HRTs are two key factors determined biomass in the photo-bioreactor and hydrogen production capacity. Moreover, the photo-fermentation hydrogen production is strongly dependent on light energy supply, the light conversion efficiency of the novel photo-bioreactor was 1.6%, it is higher than that that of most of reported photobioreactors. Therefore, these results demonstrated that the disk photo-bioreactor was a useful and efficient reactor in entire continuous hydrogen production.

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