Continuous generation and release of microplastics and nanoplastics from polystyrene by plastic-degrading marine bacteria

Abstract

Plastic waste released into the environments breaks down into microplastics due to weathering, ultraviolet (UV) radiation, mechanical abrasion, and animal grazing. However, little is known about the plastic fragmentation mediated by microbial degradation. Marine plastic-degrading bacteria may have a double-edged effect in removing plastics. In this study, two ubiquitous marine bacteria, Alcanivorax xenomutans and Halomonas titanicae, were confirmed to degrade polystyrene (PS) and lead to microplastic and nanoplastic generation. Biodegradation occurred during bacterial growth with PS as the sole energy source, and the formation of carboxyl and carboxylic acid groups, decreased heat resistance, generation of PS metabolic intermediates in cultures, and plastic weight loss were observed. The generation of microplastics was dynamic alongside PS biodegradation. The size of the released microplastics gradually changed from microsized plastics on the first day (1344 nm and 1480 nm, respectively) to nanoplastics on the 30th day (614 nm and 496 nm, respectively) by the two tested strains. The peak release from PS films reached 6.29 × 106 particles/L and 7.64 × 106 particles/L from degradation by A. xenomutans (Day 10) and H. titanicae (Day 5), respectively. Quantification revealed that 1.3% and 1.9% of PS was retained in the form of micro- and nanoplastics, while 4.5% and 1.9% were mineralized by A. xenomutans and H. titanicae at the end of incubation, respectively. This highlights the negative effects of microbial degradation, which results in the continuous release of numerous microplastics, especially nanoplastics, as a notable secondary pollution into marine ecosystems. Their fates in the vast aquatic system and their impact on marine lives are noted for further study.