Abstract
The continuous inflow of consumable substrates and outflow of products tremendously increase the lifetime of cell-free protein synthesizing systems (1). As a consequence, the protein yield of the continuous-flow cell-free (CFCF) translation can be raised up to two orders of magnitude, as compared with classical batch systems. The CFCF protein synthesis technique can be utilized for basic research in the field of protein synthesis and folding as well as for numerous applications in biotechnology.
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