Abstract
The present study focused on fermentative butyric acid production by Clostridium tyrobutyricum from pre-treated and hydrolysed wheat straw (PHWS) based on continuous operation mode and in situ acids extraction by reverse electro enhanced dialysis (REED). Different dilutions of PHWS in a synthetic medium (60–100 % v/v) were tested. It was found that continuous fermentation of PHWS greatly enhanced the sugar consumption rates and butyric acid productivity compared to batch tests, while application of REED enhanced them even further. Specifically, applying combined continuous operation mode and REED system for the fermentation of 70 % PHWS resulted in 19- and 53-fold higher glucose (1.37 g L−1 h−1) and xylose (0.80 g L−1 h−1) consumption rates, respectively, compared to those obtained by batch processing. Fermentation of 100 % PHWS continued unhindered with just urea and K2HPO4 added with butyric acid production rate, yield and selectivity being 1.30 g L−1 h−1, 0.45 g g−1 sugars and 0.88 g g−1 acids, respectively. These results were also confirmed in a 20 L pilot plant bioreactor system.
Highlights
A major step towards the development of a sustainable industrial society is a shift from petroleum-based resources to renewable resources
The present study focused on fermentative butyric acid production by Clostridium tyrobutyricum from pre-treated and hydrolysed wheat straw (PHWS) based on continuous operation mode and in situ acids extraction by reverse electro enhanced dialysis (REED)
Applying combined continuous operation mode and REED system for the fermentation of 70 % PHWS resulted in 19and 53-fold higher glucose (1.37 g L-1 h-1) and xylose (0.80 g L-1 h-1) consumption rates, respectively, compared to those obtained by batch processing
Summary
A major step towards the development of a sustainable industrial society is a shift from petroleum-based resources to renewable resources. Villadsen [4] states that this is mainly due to the fact that the large-scale equipment is very much like it was in the 1940s while ‘‘companies themselves, being so happy with the order-ofmagnitude increases in yield that are obtained by molecular biology that they overlook simpler methods for developing better production methods in large-scale’’. This approach might still be cost-efficient for production of high-value chemicals and pharmaceuticals; for production of bulk chemicals of relatively low value, other more efficient methods could potentially bring us faster to a sustainable, bio-based economy
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