Abstract
Ethanol production from oil palm frond (OPF) by simultaneous saccharification and Saccharomyces cerevisiae TISTR5048 fermentation was investigated. Solid fraction of OPF (20% TS) was pretreated by 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking at room temperature for 24 hours. Pretreated OPF by presoaking in 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 contained 37%, 42% and 49% of cellulose, respectively. Pretreated OPF was simultaneous saccharification by cellulase enzyme (Cellic CTec2, Novozymes) and sequentially fermentation. Sugar concentration in OPF cellulose hydrolysis of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 45.72, 55.73 and 56.94 g/l, respectively. Ethanol concentration of 2% H2SO4, 2% NaOH and 2% NaOH in H2O2 presoaking was 14.5, 15.0 and 17.2 g/L, respectively. 2% NaOH in H2O2 presoaking was the best pretreatment with 82.11% of total solids recovery and containing 49.9% of cellulose with enzyme digestion ability of 37.6%.
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