Continuous Decolorization of Acid Blue 62 Solution in an Enzyme Membrane Reactor.

Marcin Lewańczuk,Jolanta Bryjak

doi:10.1007/s12010-015-1741-9

Marcin Lewańczuk, Jolanta Bryjak

Open Access

https://doi.org/10.1007/s12010-015-1741-9

Copy DOI

Abstract

This paper focuses on using an enzyme membrane reactor (EMR) for the effective continuous decolorization of Acid Blue 62 (AB62). The following factors were considered for the effective use of Cerrena unicolor laccase immobilized in the EMR volume: the enzyme was stable in six successive runs in a batch reactor; no aeration was necessary; AB62 and the oxidized products were sorbed onto the membrane but were not rejected; and the enzyme was stable in the EMR system. It is obvious that any continuous process must be predictable, and thus, the objective was to verify the process model experimentally. For this reason, a proper isoenzyme kinetic equation was selected and the parameters were evaluated. The obtained kinetic parameters were used to plan processes and to verify their applicability to long-term AB62 decolorization, and a very good agreement between the calculated and the measured data was obtained. In the main designed continuous decolorization process, the conversion reached 98 % and was stable for 4 days. The membrane reactor with C. unicolor laccase appears to be very promising for AB62 decolorization.

Highlights

In recent years, with the growing concern for clean environment, more attention has been paid to the pollutants generated by textile industry
Several physicochemical [9,10,11] or biological [12,13,14,15,16] methods have been applied [8, 17, 18]. It seems that the interesting alternative for dye decolorization by biocatalyst is the application of a continuous system with a semipermeable membrane to separate products and an enzyme from a reaction mixture
The present paper focuses on the enzyme stability, substrate/product sorption onto membrane, reaction kinetics, process modeling, and the model validation in long-term Acid Blue 62 (AB62) decolorization with soluble laccase immobilized in enzyme membrane reactor (EMR)

Summary

Introduction

With the growing concern for clean environment, more attention has been paid to the pollutants generated by textile industry. Laccases can be used to treat effluents containing phenolic compounds from many industrial sectors, including textile one These treatments are based on cross-coupling reactions, homo- or hetero-polymerization, or simple degradation [5]. Several physicochemical [9,10,11] or biological [12,13,14,15,16] methods have been applied [8, 17, 18] It seems that the interesting alternative for dye decolorization by biocatalyst is the application of a continuous system with a semipermeable membrane to separate products and an enzyme from a reaction mixture

Objectives

Methods

Results

Conclusion