Abstract
Normal tissue macrophages from fetal and adult pig tissues can be continuously cultured by growing simple explant cultures on feeder layers of STO mouse embryonic fibroblasts. Macrophage cultures initiated from fetal and newborn pig testicle and liver explants grew from eight to 30 population doublings. The macrophages grew on top of the STO feeder cells in two forms: either a semi-attached round refractile morphology, or a closely attached ameboid morphology with several extended pseudopods. Cultured macrophages had large lobed nuclei, numerous complex vacuoles, and filopodia by transmission electron microscopic examination. The macrophages rapidly took up and sequestered acetylated-LDL in their vacuoles. They were highly phagocytic and expressed CD14 on their surface. Macrophage cultures were also initiated from tissues of the turkey, rat, mouse, cow, and sheep (data not shown). This simple method of isolating and propagating tissue macrophages could routinely provide macrophages for general research, adoptive immunotherapy, and somatic gene therapy.
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