Continuous cropping of alfalfa (Medicago sativa L.) reduces bacterial diversity and simplifies cooccurrence networks in aeolian sandy soil

Abstract

Alfalfa is a perennial herbaceous forage legume that is remarkably and negatively affected by monocropping. However, the contribution of the changes in bacterial communities to soil sickness in alfalfa have not been elucidated. Therefore, we investigated bacterial community structures in response to monocropped alfalfa along the chronosequence. Continuous cropping remarkably reduced bacterial alpha diversity and altered community structures, and soil pH, total P and available P were strongly associated with the changes of bacterial diversity and community structures. Intriguingly, 10 years of monocropped alfalfa might be a demarcation point separating soil bacterial community structures into two obvious groups that containing soil samples collected in less and more than 10 years. The relative abundances of copiotrophic bacteria of Actinobacteria and Gammaproteobacteria significantly increased with the extension of continuous cropping years, while the oligotrophic bacteria of Armatimonadetes, Chloroflexi, Firmicutes and Gemmatimonadetes showed the opposite changing patterns. Among those altered phyla, Actinobacteria, Chloroflexi, Alphaproteobacteria and Acidobacteria were the most important bacteria which contributed 50.86% of the community variations. Additionally, the relative abundances of nitrogen fixation bacteria of Bradyrhizobium and Mesorhizobium obviously increased with continuous cropping years, while the abundances of Arthrobacter, Bacillus, Burkholderiaceae and Microbacterium with potential functions of solubilizing phosphorus and potassium remarkably decreased after long-term continuous cropping. Furthermore, bacterial cooccurrence patterns were significantly influenced by continuous cropping years, with long-term monocropped alfalfa simplifying the complexity of the cooccurrence networks. These findings enhanced our understandings and provided references for forecasting how soil bacterial communities responds to monocropped alfalfa.