Abstract
Continuous citric acid secretion by a high specific pH dependent active transport system in yeast Candida oleophila ATCC 20177
Highlights
IntroductionExtracellular nitrogen exhaustion and entering a transition phase, and the increase in intracellular NH4+ concentration, possibly caused by proteolysis, are the most important factors influencing and triggering out citric acid formation and secretion in yeasts (Anastassiadis et al 1993; Anastassiadis 1994; Anastassiadis et al 1994; Anastassiadis et al 2001; Anastassiadis et al 2002; Anastassiadis et al 2004)
Financial support: Part of the work that has been carried out at the Institute of Biotechnology 2 of Research Centre Jülich (Germany) was financed by Haarmann and Reimer, a daughter company of the company Bayer, Leverkusen, Germany
The pH influence on continuous citric acid secretion was investigated in Candida oleophila ATCC 20177 under NH4+ limiting state steady conditions, using glucose
Summary
Extracellular nitrogen exhaustion and entering a transition phase, and the increase in intracellular NH4+ concentration, possibly caused by proteolysis, are the most important factors influencing and triggering out citric acid formation and secretion in yeasts (Anastassiadis et al 1993; Anastassiadis 1994; Anastassiadis et al 1994; Anastassiadis et al 2001; Anastassiadis et al 2002; Anastassiadis et al 2004). A negative effect on pyruvate kinase isn’t known, so there is no need for any kind of control at this point (Meixner-Monori et al 1984)
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