Abstract
Magnetically-modified Sphingomonas sp. was prepared using covalent binding of magnetic nanoparticles on to the cell surface. The magnetic modified bacteria were immobilized in the fixed-bed bioreactors (FBR) by internal and external magnetic fields for the biodetoxification of a model organophosphate, parathion: 93% of substrate (50mg parathion/l) was hydrolyzed at 0.5ml/min in internal magnetic field fixed-bed bioreactor. The deactivation rate constants (at 1ml/min) were 0.97×10(-3), 1.24×10(-3) and 4.17×10(-3)h(-1) for immobilized bacteria in external and internal magnetic field fixed-bed bioreactor and FBR, respectively. The deactivation rate constant for immobilized magnetically modified bacteria in external magnetic field fixed-bed bioreactor (EMFFBR) was 77% lower than that of immobilized cells by entrapping method on porous basalt beads in FBR at 1ml/min. Immobilized magnetic modified bacteria exhibited maximum enzyme stability in EMFFBR.
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