Abstract
Contents of gymnemic acids were investigated in 21 kinds of commercial health foods using Gymnema sylvestre leaves or extract. A hot water extract of each sample was acidified and centrifuged. The obtained precipitate was dissolved with ethanol and hydrolyzed with alkali followed by acid to hydrolyze gymnemic acid to gymnemagenin. The hydrolyzate was purified on an ODS cartridge, and gymnemagenin was measured by HPLC. The content of gymnemic acid was calculated as gymnemic acid II. Contents of gymnemic acid were N. D. (less than 10.5μg/g) to 20.5mg/g for 11 kinds of tablets, 8.46mg/g for 1 kind of powdered leaves, 2.12-6.89mg/g for 5 kinds of tea bags of “gymnema tea”, N. D. (less than 0.207μg/mL) to 4.41μg/mL for 2 kinds of soft drinks, and 0.029-0.055mg/g for 2 kinds of chewing gum.
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