Abstract
Summary The content of 5-phosphoribosyl-1-pyrophosphate (PRPP) and its availability for nucleotide synthesis changed during growth of Catharanthus roseus cells in a batch suspension culture. PRPP content varied between 0.41±0.09-2.19±0.48 nmol · g fresh weight-1 and 0.24±0.05–1.39±0.16 nmol · 107 cells −1. The content increased during the lag phase of cell growth and maximum value was obtained at the cell division phase. The rates of «PRPP availability» expressed as nmol · h−1 · g fresh weight−1 and nmol · h−1 · 107 cells −1 were 33.2±0.8–125.0±19.8 and 19.8±0.4–67.6±10.6, respectively. The maximum PRPP availability was observed in the lag phase cells. The results obtained here suggest that (i) the cellular PRPP pool is small and the turnover of PRPP is rapid in Catharanthus cells, (ii) the rates of synthesis and utilisation of PRPP increase during the lag phase of the cells, (iii) purine and pyrimidine phosphoribosyltransferases are not saturated with PRPP in the cells in vivo.
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